**Universitat de ValènciaCurriculum Vitae

BALTASAR ESCRICHE SOLER
BALTASAR ESCRICHE SOLER
E-mail baltasar.escriche(a)uv.es
Professional category CATEDRÁTICO DE UNIVERSIDAD
Department UNIV. INSTITUTE BIOTECHNOLOGY AND BIOMEDICINE (BIOTECMED)
Phone number 963543401

Journal Publications

Leopoldo Palma; Diego Herman Sauka; Baltasar Escriche (2024). Genome Sequence of Novel Bacteria Showing Potential Biotechnological Applications. Data . ISSN: 2306-5729
Leopoldo Palma; Yolanda Bel; Baltasar Escriche (2024). Genomic Insights into Bacillus thuringiensis V-CO3.3: Unveiling Its Genetic Potential against Nematodes. Data, 9(97) . https://doi.org/10.3390/data9080097 . ISSN: 2306-5729
Leopoldo Palma, Yolanda Bel, Baltasar Escriche (2024). Draft genome sequence of Bacillus thuringiensis strain V-AB8.18, a novel isolate with potential nematicidal activity. Microbiology Resource Announcements, 13(7) . https://doi.org/10.1128/mra.00227-24 . ISSN: 2576-098X
Yolanda Bel, Magda Galeano, Mireya Baños-Salmeron, Miguel Andrés-Antón, Baltasar Escriche (2024). Bacillus thuringiensis Cry5, Cry21, App6 and Xpp55 proteins to control Meloidogyne javanica and M. incognita. Applied Microbiology and Biotechnology, 108(525) . https://doi.org/10.1007/s00253-024-13365-2 . ISSN: 0175-7598
Fionna Knecht; Yolanda Bel; Dafne Toledo; Giselher Grabenweger; Baltasar Escriche (2023). Effect of Bacillus insecticidal proteins on the Japanese beetle, Popillia japonica (Scarabaeidae). Agricultural Research & Technology, 27(4), p. 556375 . https://doi.org/10.19080/ARTOAJ.2023.27.556375 . ISSN: 2471-6774
Escriche, B. (2023). Control Biotecnológico de Plagas. Phytoma España, 143(353) . ISSN: 1131-8988
Elham Jalali; Yolanda Bel; Shahab Maghsoudi; Ebrahim Noroozian; Baltasar Escriche (2023). Enhancing insecticidal efficacy of Bacillus thuringiensis Cry1Ab through pH-sensitive encapsulation. Applied Microbiology and Biotechnology, 107, pp. 6407 - 6419 . https://doi.org/10.1007/s00253-023-12723-w . ISSN: 0175-7598
Bel Y; Andrés-Antón M; Escriche B (2023). Abundance, distribution, and expression of nematicidal crystal protein genes in Bacillus thuringiensis strains from diverse habitats. International Microbiology, 26, pp. 295 - 308 . https://doi.org/10.1007/s10123-022-00307-z . ISSN: 1139-6709
Jéssica A. de Oliveira; Bárbara F. Negri; Patricia Hernández-Martínez; Marcos F. Basso; Baltasar Escriche (2023). Mpp23Aa/Xpp37Aa Insecticidal Proteins from Bacillus thuringiensis (Bacillales: Bacillaceae) Are Highly Toxic to Anthonomus grandis (Coleoptera: Curculionidae) Larvae. Toxins, 15(55) . Institutional Repository . ISSN: 2072-6651
Bel Y; Galeano M; Baños-Salmeron M; Escriche B (2022). The use of Bacillus thuringiensis to control plant-parasitic nematodes. Journal of Plant Science and Phytopathology, 6, pp. 62 - 64 . https://doi.org/10.29328/journal.jpsp.1001076 . ISSN: 2575-0135
Ayda Khorramnejad; Yolanda Bel; Reza Talaei‑Hassanloui; Baltasar Escriche (2022). Activation of Bacillus thuringiensis Cry1I to a 50 kDa stable core impairs its full toxicity to Ostrinia nubilalis. Applied Microbiology and Biotechnology(106), pp. 1745 - 1758 . Institutional Repository . ISSN: 0175-7598
Lledó Rodríguez; Patricia Hernández-Martínez; Baltasar Escriche (2022). El sistema inmune de los insectos: algo más complejo que simples barreras. Inmunologia, 41(3), pp. 9 - 19 . ISSN: 0213-9626
Álvaro Rodríguez-González ; Alejandra J. Porteous-Álvarez; Marcos Guerra; Óscar González-López; Pedro A. Casquero; Baltasar Escriche (2022). Effect of Cry toxins on Xylotrechus arvicola (Coleoptera:Cerambycidae) larvae. Insects, 13(1), p. 27 . Institutional Repository . ISSN: 2075-4450
Álvaro Rodríguez-González; Alejandra J. Porteous-Álvarez; Mario Del Val; Pedro A. Casquero; Baltasar Escriche (2021). Susceptibility of Xylotrechus arvicola (Coleoptera: Cerambycidae) to five Cry toxins. Biology, Life and Sciences Forum, 4, p. 32 . https://doi.org/10.3390/IECPS2020-08821 . ISSN: 2673-9976
Castellane, T. C. L.; Lemos, M. V.; Escriche, B. (2021). Improving Bacillus thuringiensis Toxins for Better Pest Control. Frontiers In Microbiology, 12, p. 3794 . https://doi.org/10.3389/fmicb.2021.799011 . ISSN: 1664-302X
P. Hernandez Martınez; A. Khorramnejad; K. Prentice; A. Andres-Garrido; N. M. Vera-Velasco; G. Smagghe; B. Escriche (2021). The biological activity of Bacillus thuringiensis Cry37Aa protein is impaired by biotinylation. Febs Open Bio, 11(Suppl ), p. 220 . https://doi.org/10.1002/2211-5463.13205 . ISSN: 2211-5463
Álvaro Rodríguez-González; Alejandra J. Porteous-Álvarez; Mario Del Val; Pedro A. Casquero; Baltasar Escriche (2020). Toxicity of five Cry proteins against the insect pest Acanthoscelides obtectus (Coleoptera: Chrisomelidae: Bruchinae). Journal of Invertebrate Pathology, 169, pp. 1107295-1 - 6 . Institutional Repository . ISSN: 0022-2011
Ascensión Andrés-Garrido; Rosa M. González-Martínez; Silvia Ramos; Baltasar Escriche (2020). Cadherin fragments of Lepidopteran and Coleopteran species do not enhance toxicity of Cry1Ca and Vip3Aa proteins to Spodoptera exigua (Hübner) (Lepidoptera:Noctuidae). Biocontrol Science and Technology, 30(9), pp. 941 - 950 . https://doi.org/10.1080/09583157.2020.1772960 . ISSN: 0958-3157
Domínguez-Arrizabalaga M; Villanueva M; Escriche B; Ancí­n-Azpilicueta C; Caballero P. (2020). Insecticidal Activity of Bacillus thuringiensis Proteins Against Coleopteran Pests. Toxins, 12(7), p. 430 . https://doi.org/10.3390/toxins12070430 . ISSN: 2072-6651
Ayda Khorramnejad; Mikel Domínguez-Arrizabalaga; Primitivo Caballero; Baltasar Escriche and Yolanda Bel (2020). Study of the Bacillus thuringiensis Cry1Ia Protein Oligomerization Promoted by Midgut Brush Border Membrane Vesicles of Lepidopteran and Coleopteran Insects, or Cultured Insect Cells. Toxins, 12(2), p. 133 . Institutional Repository . ISSN: 2072-6651
Khorramnejad A; Gomis-Cebolla J; Talaei-Hassanlouei R; Bel Y; Escriche B. (2020). Genomics and Proteomics Analyses Revealed Novel Candidate Pesticidal Proteins in a Lepidopteran-Toxic Bacillus thuringiensis Strain. Toxins, 12(11), p. 673 . Institutional Repository . ISSN: 2072-6651
Hernández-Martí­nez P; Khorramnejad A; Prentice K; Andrés-Garrido A; Vera- Velasco NM; Smagghe G; Escriche B. (2020). The Independent Biological Activity of Bacillus thuringiensis Cry23Aa Protein Against Cylas puncticollis. Frontiers In Microbiology, 11(1734) . Institutional Repository . ISSN: 1664-302X

Currricul@'s texts

Historial Cientifico. Baltasar Escriche is the director of the Master in Genetics and Molecular and Cellular Biology at the University of Valencia and belongs to the research group of Biotechnological Control of Pests (CBP), http://www.cbp.com.es/. He was graduated in Biology in 1985 and founded the research on the bacterium Bacillus thuringiensis in the CBP group in 1990 with the experimental work of his Ph.D. Thesis. Several aspects of Bacillus thuringiensis have been studied, with a continuous interest on discovering new bioactive proteins and improve the characterization of the already identified. New agents are essential to be prepared for resistance and to broaden the bioagents application. Currently, he applies many approaches to discover new applications from the protein, bacteria, insects, or plants, incorporating novel systems such as genomics, but without losing the basis of looking for the best alternatives to pest control. He contributed to more than 70 publications that can exemplify some of these aspects. He has participated in over 50 projects. In fact, in the last 5 years, he has participated in 12 projects funded by different sources, including different national and international public institutions, and research agreements with both national and multinational companies
Resumen investigador. Recientemente he dejado el cargo de Director del Departamento de Genética de la Universitat de Valencia tras los 2 periodos de tres años que legalmente corresponden. Por otra parte, sigo siendo Director del Master en Investigación en Biología Molecular, Celular y Genética desde 2015. Mis investigaciones autónomas comenzaron con los estudios de genética bioquímica de los pigmentos visuales de insectos, que fructificaron con la elaboración de una Tesis de Licenciatura. Posteriormente, en 1990, comenzamos a un nuevo tema relacionado unas proteínas producidas por la bacteria Bacillus thuringiensis con propiedades insecticidas y que son expresadas por la mayor parte de plantas transgénicas. Las diferentes vertientes de este tema son sobre las que he desarrollado mis investigaciones. Me he especializado en los estudios de unión de estas proteínas a receptores de los insectos, base del mecanismo de acción, llegando a estar dentro de los referentes mundiales. Este aspecto es fundamental para la prevención de la resistencia y el registro de plantas transgénicas. Actualmente, aplicamos gran número de aproximaciones al tema tanto desde la vertiente de las proteínas, las bacterias, los insectos o las plantas, incorporando sistemas novedosos como son los genómicos, pero sin perder la base de buscar las mejores alternativas al control plagas. Las publicaciones realizadas pueden ejemplificar algunos de estos aspectos. He participado en numerosos proyectos, pero hasta no obtener una posición estable no he podido figurar como investigador principal de algunos. En los últimos 6 años, he participado en 18 proyectos financiados por diferentes fuentes, incluyendo diferentes instituciones públicas nacionales e internacionales, y convenios de investigación con empresas tanto nacionales como multinacionales. Destacar los proyectos en los que he sido recientemente investigador responsable con unas cuantías muy altas y con sistemas muy exigentes de evaluación continuada como es el proyecto europeo EcoStack y el proyecto con la multinacional Dow AgroSciences
Indicadores generales de calidad de la producción. Sexenios investigación: 4 (último tramo concedido 2007-2013). En los últimos 10 años he dirigido 4 Tesis Doctorales, que obtuvieron la máxima calificación y una de ellas el premio extraordinario. Tengo publicados 71 artículos, de los cuales 4 son del presenta año 2014. Según el Google Académico, me registran 68 artículos con un numero de citas acumulado de 1105 (46 citas/año), lo que proporciona un índice h de 20 y un i10 de 28. Los datos respecto a los últimos 5 años (sin incluir 2014), han sido citados mis artículos 475 veces (22,6 citas/artículo, 95 citas/año) y que proporcionan valores de h=14 y i10=18. En este último periodo de 5 años, he publicado 21 artículos, de los cuales 12 corresponden a revista del primer cuartil (Q1), y, pese a ser tan recientes, han sido citados 149 veces (7,1 citas/artículo, 29,8 citas/año)
Articulos. 1. Bel Y; Jakubowska AK; Costa J; Herrero S; Escriche B. (2013) Comprehensive analysis of gene expression profiles of the beet armyworm Spodoptera exigua larvae challenged with Bacillus thuringiensis Vip3Aa toxin. PLoS One 8: e81927.
2. Crava CM; Farinós GP; Bel Y; Castañera P; Escriche B. (2013). Quantitative genetic analysis of Cry1Ab tolerance in Ostrinia nubilalis. Journal of Invertebrate Pathology 113: 220 - 227.
3. Hernández-Rodríguez CS; Hernández-Martínez P; Van Rie J; Escriche B; Ferré J. (2013). Shared midgut binding sites for Cry1A.105, Cry1Aa, Cry1Ab, Cry1Ac and Cry1Fa proteins from Bacillus thuringiensis in two important corn pests, Ostrinia nubilalis and Spodoptera frugiperda. PLoS One 8: e68164.
4. Hernández-Rodríguez CS; Hernández-Martínez P; Van Rie J; Escriche B; Ferré J. (2013). Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species. Applied and Environmental Microbiology. 78: 4048 - 4050.
5. Bel Y; Ferré J; Escriche B. (2011). Quantitative real-time PCR with SYBR Green detection to assess gene duplication in insects: study of gene dosage in Drosophila melanogaster (Diptera) and in Ostrinia nubilalis (Lepidoptera). BMC Research Notes. 4: 1 - 8.
6. Crava CM; Bel Y; Lee SF; Manachini B; Heckel DG; Escriche B. (2010) Study of the aminopeptidase N gene family in the lepidopterans Ostrinia nubilalis (Hübner) and Bombyx mori (L.): Sequences, mapping and expression. Insect Biochemistry and Molecular Biology. 40: 506 - 515.
7. Hernández-Martínez P; Ferré J; Escriche B. (2009) Broad-spectrum cross-resistance in Spodoptera exigua from selection with a marginally toxic Cry protein. Pest Management Science. 65: 645 - 650.
8. Y. Bel; H.A.A. Siqueira; B.D. Siegfried; J. Ferré; B. Escriche. (2009) Variability in the cadherin gene in an Ostrinia nubilalis strain selected for Cry1Ab resistance. Insect Biochemistry and Molecular Biology. 39: 218 - 223.
9. Ibiza-Palacios; M.S.; J. Ferré; S. Higurashi; K. Miyamoto; R. Sato; B. Escriche. (2008) Selective inhibition of binding of Bacillus thuringiensis Cry1Ab toxin to cadherin-like and aminopeptidase proteins in brush border membranes and dissociated epithelial cells from Bombyx mori. Biochemical Journal. 409: 215 - 221. (Reino Unido)
Proyectos destacados. 1 Competition binding of experimental proteins versus Cry1Ac and Cry1Fa from Bacillus thuringiensis using brush border membrane vesicles from Pseudoplusia includens, and Anticarsia gemmatalis larvae.
Entidad de realización: Universitat de València
Investigador/es responsable/es: Baltasar Escriche. Número de investigadores/as: 1
Entidad/es financiadora/s: Dow AgroSciences LLC Cód. según financiadora: AGI4282 RSM/nlc. Duración: 2012-2015. Cuantía total: 350.000
2 Caracterización de los receptores de membrana responsables de la toxicidad de las proteínas Cry1F, Cry2A y Vip3A de Bacillus thuringiensis
Entidad de realización: Universitat de València
Investigador responsable: Juan Ferre Manzanero. Número de investigadores/as: 5
Entidad/es financiadora/s: Generalitat Valenciana. Conselleria d'Educació i Ciència. Cód. según financiadora: PROMETEO/2011/044 Duración: 2011-2014. Cuantía total: 165.680
3 Modo de acción de proteínas insecticidas secretables de Bacillus thuringiensis.
Entidad de realización: Universitat de València
Investigador responsable: Juan Ferre Manzanero. Número de investigadores/as: 4
Entidad/es financiadora/s: Ministerio de Ciencia e Innovación. Cód. según financiadora: AGL2009-13340-C02-01. Duración: 2010-2013. Cuantía total: 268.620
4 Sweetpotato Action for Security and Health in Africa (SASHA)
Entidad de realización: Universitat de València
Investigador responsable: Baltasar Escriche Soler. Número de investigadores/as: 2
Entidad/es financiadora/s: Bill and Melinda Gates Foundation (BMGF). Duración: 2009-2014. Cuantía total: 500.000
5 Resistance to African sweetpotato weevils using biotechnology.
Entidad de realización: Universitat de València
Investigador responsable: Baltasar Escriche Soler. Número de investigadores/as: 2
Entidad/es financiadora/s: The Rockefeller Foundation. Cód. según financiadora: 08-EPP-368722-UV. Duración: 2007-2008. Cuantía total: 22.000
6 Specific binding of Bt toxins to midgut membranes from sweetpotato weevil, Cylas spp.
Entidad de realización: Universitat de València
Investigador responsable: Baltasar Escriche Soler. Número de investigadores/as: 2
Entidad/es financiadora/s: The Rockefeller Foundation. Duración: 2006. Cuantía total: 9.600
.

Baltasar Escriche is the Director of the Master in Research in Molecular and Cellular Biology, and Genetics and Secretary of the Doctoral Program in Biomedicine and Biotechnology at the University of Valencia and belongs to the BTLab team from the research group of Biotechnological Control of Pests (CBP), http://www.cbp.com.es/. He has a positive evaluation of 5 sexennial research periods and the single transference called sexennial period. Google Scholar provides for him 3504 citations, with 32 value for the h-index and 53 value for the i-index.

Several aspects of Bacillus thuringiensis have been studied, with a continuous interest in discovering new bioactive proteins and improving the characterization of the already identified. New agents are essential to be prepared for resistance and to broaden the bioagents application. The main part of the research has been focused on Lepidoptera, because of the most common activity of Bt pesticidal proteins, but research with Coleoptera has been also implemented. Understanding the mode of action of each Bt protein, conventional and new proteins, is the key to its optimal use and preventing the selection of resistant populations. He has spent more than 30 years on this purpose, and still, each day, is finding surprising aspects. He contributed to more than 80 publications mostly in open access and 150 meeting presentations that can exemplify some of these aspects. He has participated in or led over 60 projects. In fact, in the last 5 years, he has participated mostly as PI in 14 projects PI, funded by different sources, including different national and international public institutions, and research agreements with both national and multinational companies. Along this time, it has built a long body of resources, protocols, and collaborations. The problems and science are always global under some point of view, and he has been collaborating with laboratories from the whole world, including ones from the developing countries, such as in Latin America or Africa. Funds obtained from The Rockefeller Foundation, Melinda and Bill Gates Foundation, EU PRIMA program, and specific national programs to collaborate with undeveloped countries are proofs of such vocation. His involvement in the crop protection field led him to become a member of 5 scientific societies, with the special relevance of the Society for Invertebrate Pathology (SIP), where served as Secretary/Treasurer and later as President of the Bacteria Division.

Moreover, he is interested in the dissemination of the research and was co-organizer of the meetings of the SIP/IOBC societies and the one from the Spanish Society of Applied Entomology which took place in Valencia. He actively collaborates with bulletins of such societies not only the international ones but also in the national ones and provides some papers in regular journals of the agricultural sector. The implication with the international companies is evidenced by the contacts but, in addition, he has collaborations with national companies (AFRASA, S.A., PROBELTE, S.A.,¿). The contracts were to develop new materials and technology, but in other cases to offer services not present in the market. Another aspect is the technician formation in different methodologies not only by hiring in funded projects but also with tutoring in the laboratory students from regulated technical studies (Professional Formation) and taking part in specific formation actions.

He has been involved in different facets in the formation of young researches as part of his activities as a university professor, with the remarkably tutoring of 3 or 4 research works per year as Ungraduated and Master Thesis. In addition, he has participated in the evaluation commission of about 10 student research works per year from different levels: undergraduate, Master, Ph.D., but also to evaluate university professor positions. The experience allows some of them to follow in science. The doctorate students tutored by him, including several foreign researchers, obtained a high score in their Thesis and all of them have followed research careers in public and private companies or institutions.

He is a senior piece of the CBP group that has been built over decades and contributes to keeping and enlarging it. The research line of discovering new pesticide resources and the work on insect resistance has been nationally and internationally validated. In fact, he has reviewed projects from several autonomic, national, and international research agencies. It is remarkable the collaboration with the DFG (Germany), ARIS (Slovenia), and DLR (Western Balkan) agencies. Moreover, he has been invited to serve as a reviewer of more than 25 journals and has become a member sequentially to the editorial board of 5 journals. Moreover, he has participated as an invited editor for Special Issues related to B. thuringiensis in different journals.

Previous Tasks

Alumno monitor. Dep. Citología e Histología. Universitat de Valencia. 20/09/1983 - 20/09/1984
Becario de Colaboración. Dep. Genética. Universitat de Valencia. 20/09/1985 - 01/10/1987
Tesinando. Dep. Genética. Universitat de València. 01/10/1987 - 31/12/1989
Becario predoctoral. Dep. Genética. Universitat de València. 01/01/1990 - 31/05/1993
Técnico de Laboratorio. Fac. CC. Químicas. Universitat de València. 01/06/1993 - 31/08/1995
Investigador postdoctoral. Fac. Medicina. Universidad de Limburg (Bélgica). 15/09/1995 - 15/09/1997
Doctor reincorporado. Dep. Genética. Universitat de València. 15/05/1998 - 14/05/1999
Profesor Asociado 3h. Dpto. Genética. Universitat de Valencia. 18/10/1999 - 30/09/2000
Téc. Sup. Investigació. Dep. Genética. Universitat de València. 25/10/1999 - 31/12/2001
Profesor Asociado 3h. Dpto. Genética. Universitat de Valencia. 16/10/2001 - 31/12/2001

Academic training

Licenciado en CC. Biológicas . Universitat de València . 01/07/1987 . (Diploma / Degree / Activities)
Doctor en CC. Biológicas . Universitat de València . 01/07/1995 . (Ph.D.)
Grado de Licenciatura en CC. Biológicas . Universitat de València . 05/07/1990 . (Official Master)
Supervisor de Instalaciones radioactivas . Consejo Seguridad Nuclear . 20/04/2021 . (Specialized Training)

Thesis, minor thesis and research reports

Noelia Joya Carrasco . (2021) . Study of the mode of action of the Bacillus thuringiensis Cry1Aa protein in Bombyx mori (Master Thesis) . Universitat de Valencia.
Pablo Parra Hernández . (2019) . Rastreo de genes cry codificantes de proteínas con actividad nematicida en colecciones de Bacillus thuringiensis (Master Thesis) . Universitat de Valencia.
Maria Garcia Sanchez . (2019) . Actividades sinérgicas entre proteínas de Bacillus thuringiensis subsp. israelensis para el control de vectores de enfermedades (Final Degree work) . Universitat de Valencia.
Irene Gimeno Salas . (2018) . Uso biotecnológico de las proteinas de Bacillus thuringiensis para el control de insectos plaga (Final Degree work) . Universitat de Valencia.
Ayda Khoramnezhad . (2018) . Structural and functional diversity of Bacillus thuringiensis toxin on animal, plant and microbial cells (Doctoral Thesis) . University of Tehran/Universitat de València.
Sergio Torralba Pertinez . (2017) . El DNA asociado a las inclusiones cristalinas de Bacillus thuringiensis; relevancia e implicaciones (Final Degree work) . Universitat de Valencia.
Pau Chinestra Bordera . (2017) . Uso biotecnológico de las proteínas Cry de Bacillus thuringiensis para el control de los nematodos (Final Degree work) . Universitat de Valencia.
Carlos Lopez Orero . (2016) . Técnicas de PCR y diseño de cebadores para cribado de genes Cry de Bacillus thuringiensis (Final Degree work) . Universitat de Valencia.
LESLEY ESCRIVA ARDID . (2015) . Caracterización de cepas de Bacillus thuringiensis (Final Degree work) . Universidad de Valencia.
Maria de Sales Ibiza Palacios . (2015) . Bases de la resistencia a preparados insecticidas basados en Bacillus thuringiensis en diferentes especies de insectos (Doctoral Thesis) . Universitat de Valencia.